Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10: Difference between revisions
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*Zymo DNA Clean & Concentrator Kit | |||
*Add 30 μL of DNA Binding Buffer to each 15 μL PCR Reaction (#1,2,3,4,5) | |||
*Pipette up and down mixture several times to make sure it's thoroughly mixed. | |||
*Centrifuged at top speed for 30 seconds. Flow through was discarded. | |||
*Added 200 μL of Wash Buffer the column and centrifuged for 30 seconds. Repeated step. | |||
*Spin Column for each PCR rxn placed in new 1.5 mL tube. | |||
*30μL of water was direct added to the column to elute DNA. | |||
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Revision as of 01:28, 14 December 2012
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Summary
Follow steps for gel electrophoresis.
PCR of Gibson BioBricks
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