Haynes Lab:Notebook/Engineering PC-TFs/2012/12/07

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(Summary)
(Summary)
Line 10: Line 10:
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*Assemblies
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1. hPCD - Pflex - BL01<br>
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2. hPCD - BL01
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{|border="1" cellpadding="5" cellspacing="0"
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|-
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! scope="col" style="background:#efefef;" | #
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! scope="col" style="background:#efefef;" | Template
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! scope="col" style="background:#efefef;" | Primers
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|-
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|1
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|hPCD
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|9, 2
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|-
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|2
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|Plflex
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|3, 4
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|-
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|3
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|BL01
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|5,10
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|-
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|4
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|hPCD
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|9, 7
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|-
 +
|5
 +
|BL01
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|8,10
 +
|-
 +
|}
 +
 +
{|border="1" cellpadding="5" cellspacing="0"
 +
|-
 +
! scope="col" style="background:#efefef;" |
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! scope="col" style="background:#efefef;" | μL
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|-
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|Template
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|.5 μL
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|-
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|Primer 1 (10 μm)
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|1.0 μL
 +
|-
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|Primer 2 (10 μm)
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|1.0 μL
 +
|-
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|2*GoTaq
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|12. 5
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|-
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|DH2O
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|10.0 μL
 +
|-
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|}
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PCR was set with preset settings:
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 +
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Revision as of 18:54, 7 December 2012

Engineering PC-TFs Main project page
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Summary

  • Performed another PCR run because lid was not closed entirely yesterday. Next time in lab(Monday), confirmation gel will be run to show that PCR rxns worked.


  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01

# Template Primers
1 hPCD 9, 2
2 Plflex 3, 4
3 BL01 5,10
4 hPCD 9, 7
5 BL01 8,10
μL
Template .5 μL
Primer 1 (10 μm) 1.0 μL
Primer 2 (10 μm) 1.0 μL
2*GoTaq 12. 5
DH2O 10.0 μL

PCR was set with preset settings:



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