Haynes Lab:Notebook/Engineering PC-TFs/2012/12/07

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*Ran Zymoclean DNA recovery kit.
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Revision as of 19:00, 7 December 2012

Engineering PC-TFs Main project page
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Summary

  • Performed another PCR run because lid was not closed entirely yesterday. Next time in lab(Monday), confirmation gel will be run to show that PCR rxns worked.


  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01

# Template Primers
1 hPCD 9, 2
2 Plflex 3, 4
3 BL01 5,10
4 hPCD 9, 7
5 BL01 8,10
μL
Template .5 μL
Primer 1 (10 μm) 1.0 μL
Primer 2 (10 μm) 1.0 μL
2*GoTaq 12. 5
DH2O 10.0 μL

PCR was set with preset settings:

  • Ran Zymoclean DNA recovery kit.


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