Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06

Summary

Dilute primers that came from IDT (10x dilution of concentration)

• BL_Primer09 26.6 nm --> 266 μL dilution
• BL_Primer10 25.7 nm --> 257 μL dilution

• Thaw and dilute primers that came (x10 dilution)
• 90 μL water
• 10 μL primer stock

(10 μm in each tube for PCR reaction)
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• Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01

Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions.
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*Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert

• Vi helped me with running gel because I had class. Vi will put digest into gel and will get results.

• I believe that I did not wait long enough after I microwaved the gel to put in the ethnium bromide. I need to be sure that I wait until its cooled down.

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PSB1A3 VECTOR digest

• Vi cut gel and has it available for me to work on tomorrow afternoon.
• Vi also took out my PCR reaction. She told me that the lid was not closed all the way, so I may need to redo rxns.