Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06: Difference between revisions
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Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions. | Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions.<br> | ||
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*Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert | *Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert | ||
Revision as of 16:20, 6 December 2012
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||
SummaryDilute primers that came from IDT (10x dilution of concentration)
(10 μm in each tube for PCR reaction)
1. hPCD - Pflex - BL01
Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions. *Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert
Vi helped me with running gel because I had class. Vi will put digest into gel and will get results.
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