Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06

Summary

Dilute primers that came from IDT (10x dilution of concentration)

• BL_Primer09 26.6 nm --> 266 μL dilution
• BL_Primer10 25.7 nm --> 257 μL dilution

• Thaw and dilute primers that came (x10 dilution)
• 90 μL water
• 10 μL primer stock

(10 μm in each tube for PCR reaction)
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• Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01

*Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert

Vi helped me with running gel because I had class. Vi will put digest into gel and will get results.