Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06: Difference between revisions

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1. hPCD - Pflex - BL01<br>
1. hPCD - Pflex - BL01<br>
2. hPCD - BL01
2. hPCD - BL01
{|border="1" cellpadding="5" cellspacing="0" align=";left"
|-
! scope="col" style="background:#efefef;" | #
! scope="col" style="background:#efefef;" | Template
! scope="col" style="background:#efefef;" | Primers
|-
|1
|hPCD
|9, 2
|-
|2
|Plflex
|3, 4
|-
|3
|BL01
|5,10
|-
|4
|hPCD
|9, 7
|-
|5
|BL01
|8,10
|-
|}
{|border="1" cellpadding="5" cellspacing="0" align="left"
|-
! scope="col" style="background:#efefef;" |
! scope="col" style="background:#efefef;" | μL
|-
|Template
|.5 μL
|-
|Primer 1 (10 μm)
|1.0 μL
|-
|Primer 2 (10 μm)
|1.0 μL
|-
|2*GoTaq
|12. 5
|-
|DH2O
|10.0 μL
|-
|}




Revision as of 15:55, 6 December 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

Dilute primers that came from IDT (10x dilution of concentration)

  • BL_Primer09 26.6 nm --> 266 μL dilution
  • BL_Primer10 25.7 nm --> 257 μL dilution
  • Thaw and dilute primers that came (x10 dilution)
  • 90 μL water
  • 10 μL primer stock

(10 μm in each tube for PCR reaction)

  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01

# Template Primers
1 hPCD 9, 2
2 Plflex 3, 4
3 BL01 5,10
4 hPCD 9, 7
5 BL01 8,10
μL
Template .5 μL
Primer 1 (10 μm) 1.0 μL
Primer 2 (10 μm) 1.0 μL
2*GoTaq 12. 5
DH2O 10.0 μL




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