Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06

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(Summary)
(Summary)
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1. hPCD - Pflex - BL01<br>
1. hPCD - Pflex - BL01<br>
2. hPCD - BL01
2. hPCD - BL01
 +
 +
{|border="1" cellpadding="5" cellspacing="0" align=";left"
 +
|-
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! scope="col" style="background:#efefef;" | #
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! scope="col" style="background:#efefef;" | Template
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! scope="col" style="background:#efefef;" | Primers
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|-
 +
|1
 +
|hPCD
 +
|9, 2
 +
|-
 +
|2
 +
|Plflex
 +
|3, 4
 +
|-
 +
|3
 +
|BL01
 +
|5,10
 +
|-
 +
|4
 +
|hPCD
 +
|9, 7
 +
|-
 +
|5
 +
|BL01
 +
|8,10
 +
|-
 +
|}
 +
 +
{|border="1" cellpadding="5" cellspacing="0" align="left"
 +
|-
 +
! scope="col" style="background:#efefef;" |
 +
! scope="col" style="background:#efefef;" | μL
 +
|-
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|Template
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|.5 μL
 +
|-
 +
|Primer 1 (10 μm)
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|1.0 μL
 +
|-
 +
|Primer 2 (10 μm)
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|1.0 μL
 +
|-
 +
|2*GoTaq
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|12. 5
 +
|-
 +
|DH2O
 +
|10.0 μL
 +
|-
 +
|}
 +

Revision as of 17:55, 6 December 2012

Engineering PC-TFs Main project page
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Summary

Dilute primers that came from IDT (10x dilution of concentration)

  • BL_Primer09 26.6 nm --> 266 μL dilution
  • BL_Primer10 25.7 nm --> 257 μL dilution
  • Thaw and dilute primers that came (x10 dilution)
  • 90 μL water
  • 10 μL primer stock

(10 μm in each tube for PCR reaction)

  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01

# Template Primers
1 hPCD 9, 2
2 Plflex 3, 4
3 BL01 5,10
4 hPCD 9, 7
5 BL01 8,10
μL
Template .5 μL
Primer 1 (10 μm) 1.0 μL
Primer 2 (10 μm) 1.0 μL
2*GoTaq 12. 5
DH2O 10.0 μL




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