Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06
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==Summary== | ==Summary== | ||
| - | * | + | |
| + | Dilute primers that came from IDT (10x dilution of concentration) | ||
| + | |||
| + | BL_Primer09 26.6 nm --> 266 μL dilution | ||
| + | BL_Primer10 25.7 nm --> 257 μL dilution | ||
| + | |||
| + | *Thaw and dilute primers that came (x10 dilution) | ||
| + | *90 μL water | ||
| + | *10 μL primer stock | ||
| + | |||
| + | (10 μm in each tube for PCR reaction) | ||
| + | |||
| + | *Assemblies | ||
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| + | 1. hPCD - Pflex - BL01<br> | ||
| + | 2. hPCD - BL01 | ||
| + | |||
Revision as of 18:27, 6 December 2012
 Engineering PC-TFs
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SummaryDilute primers that came from IDT (10x dilution of concentration) BL_Primer09 26.6 nm --> 266 μL dilution BL_Primer10 25.7 nm --> 257 μL dilution
(10 μm in each tube for PCR reaction)
1. hPCD - Pflex - BL01
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