Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 74: | Line 74: | ||
|} | |} | ||
Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> || | |||
| rowspan="9" | | |||
|- | |||
| DNA (plasmid) || 20.0 | |||
|- | |||
| 10x buffer || 3.0 | |||
|- | |||
| XbaI || 1.0 | |||
|- | |||
| SpeI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 5.0 | |||
|- | |||
| || 30 μL --> 37°C/ ~10 min. | |||
|} | |||
Vi helped me with running gel because I had class. Vi will put digest into gel and will get results. | |||
Revision as of 16:17, 6 December 2012
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||
SummaryDilute primers that came from IDT (10x dilution of concentration)
(10 μm in each tube for PCR reaction)
1. hPCD - Pflex - BL01
Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert
Vi helped me with running gel because I had class. Vi will put digest into gel and will get results.
|