Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06: Difference between revisions
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Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions. | Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions.<br> | ||
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*Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert | *Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert | ||
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Vi helped me with running gel because I had class. Vi will put digest into gel and will get results. | *Vi helped me with running gel because I had class. Vi will put digest into gel and will get results. | ||
*''I believe that I did not wait long enough after I microwaved the gel to put in the ethnium bromide. I need to be sure that I wait until its cooled down.'' | |||
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[[Image:December_6_2012.jpg|400px|thumb|PSB1A3 VECTOR digest]] | |||
*Vi cut gel and has it available for me to work on tomorrow afternoon. | |||
*Vi also took out my PCR reaction. She told me that the lid was not closed all the way, so I may need to redo rxns. | |||
Revision as of 18:32, 6 September 2013
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SummaryDilute primers that came from IDT (10x dilution of concentration)
(10 μm in each tube for PCR reaction)
1. hPCD - Pflex - BL01
Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions. *Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert
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