Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06: Difference between revisions
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(10 μm in each tube for PCR reaction) | (10 μm in each tube for PCR reaction) | ||
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*Assemblies | *Assemblies | ||
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2. hPCD - BL01 | 2. hPCD - BL01 | ||
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Revision as of 16:18, 6 December 2012
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SummaryDilute primers that came from IDT (10x dilution of concentration)
(10 μm in each tube for PCR reaction) ______________________________________
1. hPCD - Pflex - BL01
*Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert
Vi helped me with running gel because I had class. Vi will put digest into gel and will get results.
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