Haynes Lab:Notebook/Engineering PC-TFs/2012/11/29: Difference between revisions
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*Transfer the column into a clean 1.5 ml microcentrifuge tube then add 30 μl of Zyppy Elution Buffer directly to the column matrix and let stand for one min at room temperature Centrifuge for 15 seconds to elute the DNA. | *Transfer the column into a clean 1.5 ml microcentrifuge tube then add 30 μl of Zyppy Elution Buffer directly to the column matrix and let stand for one min at room temperature Centrifuge for 15 seconds to elute the DNA. | ||
Plan for tomorrow: Perform | Plan for tomorrow: Perform plasmid digest on psb1a2 vector. If digest does not succeed, will streak plasmid bacteria on plate. I will ask Vi for digest DNA. | ||
Revision as of 19:10, 29 November 2012
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Summary
Plan for tomorrow: Perform plasmid digest on psb1a2 vector. If digest does not succeed, will streak plasmid bacteria on plate. I will ask Vi for digest DNA.
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