Haynes Lab:Notebook/Engineering PC-TFs/2012/11/08 - Revision history

Brady S. Laughlin: /* Summary */

2012-11-12T00:16:48Z

Summary

←Older revision		Revision as of 00:16, 12 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Summary==		==Summary==
-	<i>Assembly Check of PCR Reactions 1,2,3,4~~<i>~~	+	<i>Assembly Check of PCR Reactions 1,2,3,4

	* Gel Electrophoresis		* Gel Electrophoresis
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	*Turn on electrophoresis and let run for thirty minutes.		*Turn on electrophoresis and let run for thirty minutes.

-	<b>The assembly did not work. No bands were seen when placed under UV light.~~<b>~~	+	<b>The assembly did not work. No bands were seen when placed under UV light.

Brady S. Laughlin: /* Summary */

2012-11-12T00:16:26Z

Summary

←Older revision		Revision as of 00:16, 12 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Summary==		==Summary==
-	Assembly Check of PCR Reactions 1,2,3,4<i>	+	<i>Assembly Check of PCR Reactions 1,2,3,4<i>

	* Gel Electrophoresis		* Gel Electrophoresis
Line 24:		Line 24:
	*Turn on electrophoresis and let run for thirty minutes.		*Turn on electrophoresis and let run for thirty minutes.

-	The assembly did not work. No bands were seen when placed under UV light.<b>	+	<b>The assembly did not work. No bands were seen when placed under UV light.<b>

Brady S. Laughlin: /* Summary */

2012-11-12T00:15:51Z

Summary

←Older revision		Revision as of 00:15, 12 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Summary==		==Summary==
-	*	+	Assembly Check of PCR Reactions 1,2,3,4<i>
		+
		+	* Gel Electrophoresis
		+
		+	Follow steps for gel electrophoresis.
		+	*Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray.
		+	*Create 1% gel by putting .6 grams of agarose into flask.
		+	*Microwave agarose solution for 30 seconds
		+	*Using hot gloves, grab flask out of microwave and swirl flask vigorously. Place back in microwave for 30 more seconds.
		+	*When flask is taken out of microwave, make sure that the agarose is completed dissolved.
		+	*Set up wells on gel electrophoresis apparatus. The mold for the first digest is B-10. Use the thin "teeth" and slip in the notches.
		+	*Add EtBr (ethidium bromide) to the agarose gel. There should be 1 microliter of EtBr per 10ml of agarose gel. Therefore, 6 µL.
		+	*Pour gel into tray. Wait twenty minutes for gel to settle.
		+	*Wash the agarose gel flask.
		+	*Pipette 15 μL of DNA ladder into first well. Pipette 5μL of PCR reactions into subsequent wells.
		+	*Turn on electrophoresis and let run for thirty minutes.
		+
		+	The assembly did not work. No bands were seen when placed under UV light.<b>
		+

Brady S. Laughlin: Autocreate 2012/11/08 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs

2012-11-12T00:13:47Z

Autocreate 2012/11/08 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs

New page

{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Engineering PC-TFs</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Summary==
*


|}

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