Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06

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(Summary)
(Summary)
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{|border="1" cellpadding="5" cellspacing="0" align="left"
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|-
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! scope="col" style="background:#efefef;" | <i>DNA fragments</i>
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! scope="col" style="background:#efefef;" | 1
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! scope="col" style="background:#efefef;" | 2
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|-
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|#1
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|1.0 μL
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|
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|-
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|#2
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|1.0 μL
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|
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|-
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|#3
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|1.0 μL
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|
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|-
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|#4
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|
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|1.0 μL
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|-
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|#5
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|
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|1.0 μL
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|-
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|dH2O
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|2.0 μL
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|3.0 μL
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|-
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|<i>Total</i>
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|5.0 μL
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|5.0 μL
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|}
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Revision as of 19:24, 6 November 2012

Engineering PC-TFs Main project page
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Summary

  • Gibson Assembly

Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.

1 2
Gibson Rxn mix 15 μL 15 μL
DNA fragments 5 μL 5 μL





DNA fragments 1 2
#1 1.0 μL
#2 1.0 μL
#3 1.0 μL
#4 1.0 μL
#5 1.0 μL
dH2O 2.0 μL 3.0 μL
Total 5.0 μL 5.0 μL






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