Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06
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==Summary== | ==Summary== | ||
| - | * | + | * <u>Gibson Assembly </u> |
| + | |||
| + | Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. | ||
| + | There is no addition of vector this time. | ||
| + | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
| + | |- | ||
| + | ! scope="col" style="background:#efefef;" | | ||
| + | ! scope="col" style="background:#efefef;" | 1 | ||
| + | ! scope="col" style="background:#efefef;" | 2 | ||
| + | |||
| + | |- | ||
| + | |Gibson Rxn mix | ||
| + | |15 μL | ||
| + | |15 μL | ||
| + | |- | ||
| + | |DNA fragments | ||
| + | |5 μL | ||
| + | |5 μL | ||
| + | |} | ||
| + | |||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
Revision as of 19:23, 6 November 2012
 Engineering PC-TFs
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Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.
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