Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06

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(Autocreate 2012/11/06 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
(Summary)
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==Summary==
==Summary==
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* <u>Gibson Assembly </u>
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Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.
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There is no addition of vector this time.
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{|border="1" cellpadding="5" cellspacing="0" align="left"
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|-
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! scope="col" style="background:#efefef;" |
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! scope="col" style="background:#efefef;" | 1
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! scope="col" style="background:#efefef;" | 2
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|-
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|Gibson Rxn mix
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|15 μL
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|15 μL
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|-
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|DNA fragments
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|5 μL
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|5 μL
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|}
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<br>
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<br>
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<br>

Revision as of 18:23, 6 November 2012

Engineering PC-TFs Main project page
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Summary

  • Gibson Assembly

Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.

1 2
Gibson Rxn mix 15 μL 15 μL
DNA fragments 5 μL 5 μL






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