Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06: Difference between revisions

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{|border="1" cellpadding="5" cellspacing="0" align="left"
|-
! scope="col" style="background:#efefef;" | <i>DNA fragments</i>
! scope="col" style="background:#efefef;" | 1
! scope="col" style="background:#efefef;" | 2
|-
|#1
|1.0 μL
|
|-
|#2
|1.0 μL
|
|-
|#3
|1.0 μL
|
|-
|#4
|
|1.0 μL
|-
|#5
|
|1.0 μL
|-
|dH2O
|2.0 μL
|3.0 μL
|-
|<i>Total</i>
|5.0 μL
|5.0 μL
|}
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Revision as of 16:24, 6 November 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Gibson Assembly

Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.

1 2
Gibson Rxn mix 15 μL 15 μL
DNA fragments 5 μL 5 μL





DNA fragments 1 2
#1 1.0 μL
#2 1.0 μL
#3 1.0 μL
#4 1.0 μL
#5 1.0 μL
dH2O 2.0 μL 3.0 μL
Total 5.0 μL 5.0 μL






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