Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06: Difference between revisions
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==Summary== | ==Summary== | ||
* <u>Gibson Assembly </u> | * <u>Gibson Assembly </u> | ||
* Check with PCR | |||
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. | Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. | ||
| Line 13: | Line 14: | ||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
|- | |- | ||
! scope="col" style="background: | ! scope="col" style="background:grey;" | | ||
! scope="col" style="background:#efefef;" | 1 | ! scope="col" style="background:#efefef;" | 1 | ||
! scope="col" style="background:#efefef;" | 2 | ! scope="col" style="background:#efefef;" | 2 | ||
| Line 38: | Line 39: | ||
! scope="col" style="background:#efefef;" | <i>DNA fragments</i> | ! scope="col" style="background:#efefef;" | <i>DNA fragments</i> | ||
! scope="col" style="background:#efefef;" | 1 | ! scope="col" style="background:#efefef;" | 1 | ||
! scope="col" style="background:#efefef;" | 2 | |||
|- | |||
|#1 | |||
|1.0 μL | |||
| | |||
|- | |||
|#2 | |||
|1.0 μL | |||
| | |||
|- | |||
|#3 | |||
|1.0 μL | |||
| | |||
|- | |||
|#4 | |||
| | |||
|1.0 μL | |||
|- | |||
|#5 | |||
| | |||
|1.0 μL | |||
|- | |||
|dH2O | |||
|2.0 μL | |||
|3.0 μL | |||
|- | |||
|<i>Total</i> | |||
|5.0 μL | |||
|5.0 μL | |||
|} | |||
<br> | |||
---- | |||
PCR Check | |||
# Product 1: outer-most primers for whole assembly | |||
# Product 1: Gibson primer pair for hPCD | |||
# Product 2: outer-most primers | |||
# Product 2: Gibson primer pair for hPCD | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | |||
|- | |||
| <i>Reagent</i> || scope="col" style="background:#efefef;" | 1 | |||
! scope="col" style="background:#efefef;" | 2 | ! scope="col" style="background:#efefef;" | 2 | ||
Revision as of 16:48, 6 November 2012
 Engineering PC-TFs
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.
PCR Check
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