Haynes Lab:Notebook/Engineering PC-TFs/2012/10/26

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Summary

Assembly # Concentration
1 24.41 ng/μL
2 17.025 ng/μL
3 42.325 ng/μL
4 16.706 ng/μL
5 45.015 ng/μL











  • Gibson Assembly

Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.

1 2
Gibson Rxn mix 15 μL 15 μL
DNA fragments 5 μL




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