Haynes Lab:Notebook/Engineering PC-TFs/2012/10/26: Difference between revisions
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|DNA fragments | |DNA fragments | ||
|5 μL | |||
|5 μL | |5 μL | ||
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{|border="1" cellpadding="5" cellspacing="0" align="left" | |||
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! scope="col" style="background:#efefef;" | <i>DNA fragments</i> | |||
! scope="col" style="background:#efefef;" | 1 | |||
! scope="col" style="background:#efefef;" | 2 | |||
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|#1 | |||
|1.0 μL | |||
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|#2 | |||
|1.0 μL | |||
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|1.0 μL | |||
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|#4 | |||
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|1.0 μL | |||
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|#5 | |||
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|1.0 μL | |||
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|Vector | |||
|1.0 μL | |||
|1.0 μL | |||
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|dH2O | |||
|1.0 μL | |||
|2.0 μL | |||
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|<i>Total</i> | |||
|5.0 μL | |||
|5.0 μL | |||
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*Thaw DHSα Turbo cells (50 μL per transformation) | |||
*Add 20 μL of Gibson reaction to 50μL DHSα Turbo. | |||
**Assembly 1 | |||
**Assembly 2 | |||
**Negative Control (DH2O) | |||
*Incubate on ice for five minutes. | |||
*Plate 70 μL transformation on warm Amp plates. | |||
Revision as of 13:39, 30 October 2012
 Engineering PC-TFs
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.
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