Haynes Lab:Notebook/Engineering PC-TFs/2012/10/02

From OpenWetWare

< Haynes Lab:Notebook | Engineering PC-TFs | 2012 | 10(Difference between revisions)
Jump to: navigation, search
(→Summary)
Current revision (12:54, 3 October 2012) (view source)
(→Summary)
 
Line 24: Line 24:
-
[[Image:October_2_gel|400px|thumb|left|PCR Check]]
+
[[Image:October_2_gel.jpg|400px|thumb|left|PCR Check]]
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Engineering PC-TFs Main project page
Previous entry      Next entry

Summary

  • Gel Electrophoresis

Follow steps for gel electrophoresis.

  • Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray.
  • Create 1% gel by putting .6 grams of agarose into flask.
  • Microwave agarose solution for 30 seconds
  • Using hot gloves, grab flask out of microwave and swirl flask vigorously. Place back in microwave for 30 more seconds.
  • When flask is taken out of microwave, make sure that the agarose is completed dissolved.
  • Set up wells on gel electrophoresis apparatus. The mold for the first digest is B-10. Use the thin "teeth" and slip in the notches.
  • Add EtBr (ethidium bromide) to the agarose gel. There should be 1 microliter of EtBr per 10ml of agarose gel. Therefore, 6 µL.
  • Pour gel into tray. Wait twenty minutes for gel to settle.
  • Wash the agarose gel flask.
  • Pipette 15 μL of DNA ladder into first well. Pipette 5μL of PCR reactions into subsequent wells.
  • Turn on electrophoresis and let run for thirty minutes.


PCR Check
PCR Check


Retrieved from "http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs/2012/10/02"
Personal tools