Haynes Lab:Notebook/Engineering PC-TFs/2012/04/16
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Follow steps for gel electrophoresis.
I believe that I made some mistakes when pipetting the DNA into the wells in the gel. I'm going to run another diagnostic digest to confirm whether my ligations were successful or not. For this diagnostic digest, the number of base pairs are not matching up. But the bands for 1.1 and 1.2 are not there so I believe that I made some mistakes.