Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/Entry Base: Difference between revisions
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==== Steps for Transformation ==== | ==== Steps for Transformation ==== | ||
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Using KAH06 for Plasmid.<br> | Using KAH06 for Plasmid.<br> | ||
Take 1 μL of KAH06.<br> | Take 1 μL of KAH06.<br> | ||
Add 9 μL of ddH20. <br> | Add 9 μL of ddH20. <br> | ||
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=====Notes===== | |||
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*KAH06 has a bp length of 1170. The gene codes for hCBX8, a human chromobox protein (8). | |||
==Friday, June 28th== | ==Friday, June 28th== |
Revision as of 12:55, 30 June 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
Tuesday, June 25thSteps for TransformationUsing KAH06 for Plasmid. Notes
Friday, June 28thMiniprepAims: Run plasmids through miniprep in order to isolate plasmid DNA and determine concentration. Protocol: A. Pre-Miniprep1. Add 3 mL LB into 15 mL tube B. Miniprep1. Pipette 1.5 mL of sample into 2 mL tube. Spin for 3 min @ max setting (13.1g). Repeat once more to spin entire sample. C. DNA Concentration- Take 3 Plate1. Open program Gen 5 2.0 before machine. Results
Causes of Errors
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