Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27: Difference between revisions
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| Rxn 4: || treated cells || PCSK1 | | Rxn 4: || treated cells || PCSK1 | ||
|- | |- | ||
| Rxn 5: || treated cells || | | Rxn 5: || treated cells || IAPP | ||
|- | |- | ||
| Rxn 6: || | | Rxn 6: || treated cells || KCNQ2 | ||
|- | |- | ||
| Rxn 7: || | | Rxn 7: || treated cells || GAPD (reference gene) | ||
|- | |- | ||
| Rxn 8: || untreated cells || | | Rxn 8: || untreated cells || PDX1 | ||
|- | |- | ||
| Rxn 9: || untreated cells || | | Rxn 9: || untreated cells || MAFA | ||
|- | |- | ||
| Rxn 10: || untreated cells || | | Rxn 10: || untreated cells || GLP1R | ||
|- | |- | ||
| Rxn 11: || | | Rxn 11: || untreated cells || PCSK1 | ||
|- | |- | ||
| Rxn 12: || | | Rxn 12: || untreated cells || IAPP | ||
|- | |- | ||
| Rxn 13: || | | Rxn 13: || untreated cells || KCNQ2 | ||
|- | |- | ||
| Rxn 14: || | | Rxn 14: || untreated cells || GAPD (reference gene) | ||
|- | |- | ||
| Rxn 15: || no template || GAPD (reference gene) | | Rxn 15: || no template || PDX1 | ||
|- | |||
| Rxn 16: || no template || MAFA | |||
|- | |||
| Rxn 17: || no template || GLP1R | |||
|- | |||
| Rxn 18: || no template || PCSK1 | |||
|- | |||
| Rxn 19: || no template || IAPP | |||
|- | |||
| Rxn 20: || no template || KCN2Q | |||
|- | |||
| Rxn 21: || no template || GAPD (reference gene) | |||
|} | |} | ||
*Run 3 Each | |||
*45 Reactions Run | |||
*Using Variation 2 | |||
Variation 2<br> [[Image:Haynes_UPL_fig2.png|300px|Figure 1]] | |||
*Using as Reference, discount gene names on left side | |||
==Primers== | |||
*Created from Roche Applied Science https://www.roche-applied-science.com/shop/CategoryDisplay?catalogId=10001&tab=&identifier=Universal+Probe+Library&langId=-1#tab-3 | |||
{| class="wikitable" style="width: 800px;" | |||
|- valign="top" | |||
| '''PRIMERS LIST''' | |||
{| width= 700px | |||
|- | |||
| || '''Roche Name''' || '''Left Primer''' || '''Right Primer''' || '''UPL probe''' | |||
|- | |||
| PDX1 || NM_000209.3 || aagctcacgcgtggaaag || gccgtgagatgtacttgttgaa || #78, cat.no. 04689011001 | |||
|- | |||
| MAFA || NM_201589.3 || agcgagaagtgccaactcc || ttgtacaggtcccgctcttt || #39, cat.no. 04687973001 | |||
|- | |||
| GLP1R || NM_002062.3 || gtggcggccaattactactg || ggccagcagtgtgtacagg || #22, cat.no. 04686969001 | |||
|- | |||
| PCSK1 || NM_000439.4 || caagagcttgtgaaggacaaga || tctttcagccaagagcacag || #1, cat.no. 04684974001 | |||
|- | |||
| IAPP || NM_000415.2 || ttaccaaattgtagaggctttcg || ccctgcctctatacactcactacc || #77, cat.no. 04689003001 | |||
|- | |||
| KCNQ2(4) || NM_172108.3 || gacgtcatcgagcagtactca || cccacgatctggtccact || #56, cat.no. 04688538001 | |||
|- | |||
| GAPD (Ref) || NM_002046.3 || agccacatcgctcagacac || gcccaatacgaccaaatcc || #60, cat.no. 04688589001 | |||
|} | |||
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|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 00:13, 15 November 2013
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UPLassay
Reaction List
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