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 Haynes BioBrick Cloning
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UPLassay
- This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.
Reaction List
REACTION LIST
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Template cDNA |
Gene Target
|
| Rxn 1: |
treated cells |
PDX1
|
| Rxn 2: |
treated cells |
MAFA
|
| Rxn 3: |
treated cells |
GLP1R
|
| Rxn 4: |
treated cells |
PCSK1
|
| Rxn 5: |
treated cells |
GAPD (reference gene)
|
| Rxn 6: |
untreated cells |
PDX1
|
| Rxn 7: |
untreated cells |
MAFA
|
| Rxn 8: |
untreated cells |
GLP1R
|
| Rxn 9: |
untreated cells |
PCSK1
|
| Rxn 10: |
untreated cells |
GAPD (reference gene)
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| Rxn 11: |
no template |
PDX1
|
| Rxn 12: |
no template |
MAFA
|
| Rxn 13: |
no template |
GLP1R
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| Rxn 14: |
no template |
PCSK1
|
| Rxn 15: |
no template |
GAPD (reference gene)
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- Run 3 Each
- 45 Reactions Run
- Using Variation 2
Variation 2
[[Image:Haynes_UPL_fig2.png|300px|Figure 1]
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Haynes BioBrick Cloning
