Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27

From OpenWetWare
Jump to navigationJump to search
Haynes BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

UPLassay

  • This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.

Reaction List

REACTION LIST
  Template cDNA Gene Target
Rxn 1: treated cells PDX1
Rxn 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells GAPD (reference gene)
Rxn 6: untreated cells PDX1
Rxn 7: untreated cells MAFA
Rxn 8: untreated cells GLP1R
Rxn 9: untreated cells PCSK1
Rxn 10: untreated cells GAPD (reference gene)
Rxn 11: no template PDX1
Rxn 12: no template MAFA
Rxn 13: no template GLP1R
Rxn 14: no template PCSK1
Rxn 15: no template GAPD (reference gene)




Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/David_Barclay_Undergrad_Training/Plasmid_Transformation/2013/10/27&oldid=750983"