Haynes Lab:Notebook/Chimera Biosensor (C.R.SYS)/Entry Base

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(Autocreated Lab Notebook name=Haynes Lab:Notebook/Chimera Biosensor (C.R.SYS)/Entry Base, content from MediaWiki:EntryContentDefault)
Current revision (13:55, 19 February 2013) (view source)
(Entry title)
 
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==Entry title==
==Entry title==
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* Insert content here...
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<!-- Example table -->
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'''Minipreps'''<br>
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* Check with E/P digests
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{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
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|- valign="top"
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| bgcolor=#cfcfcf | Reagent
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| bgcolor=#cfcfcf | Volume
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| rowspan="7" | <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>
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| rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
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|-
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| DNA(plasmid) || 2.0 μL
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|-
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| 10X buffer || 1.5
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|-
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| EcoRI || 1.0
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|-
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| PstI || 1.0
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|-
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| dH<sub>2</sub>O || 9.5
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|-
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| &nbsp; || 15 μL --> 37°C/ ~15 min.
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|}

Current revision

Project name Main project page

Entry title

Minipreps

  • Check with E/P digests
Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



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