Haynes Lab:Notebook/Chimera Biosensor (C.R.SYS)/Entry Base: Difference between revisions

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(Autocreated Lab Notebook name=Haynes Lab:Notebook/Chimera Biosensor (C.R.SYS)/Entry Base, content from MediaWiki:EntryContentDefault)
 
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==Entry title==
==Entry title==
* Insert content here...
 
<!-- Example table -->
 
'''Minipreps'''<br>
* Check with E/P digests
 
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>
| rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
|-
| DNA(plasmid) || 2.0 μL
|-
| 10X buffer || 1.5
|-
| EcoRI || 1.0
|-
| PstI || 1.0
|-
| dH<sub>2</sub>O || 9.5
|-
| &nbsp; || 15 μL --> 37°C/ ~15 min.
|}




Revision as of 10:55, 19 February 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Entry title

Minipreps

  • Check with E/P digests
Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



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