Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/06/28: Difference between revisions
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<font color = purple> | <font color = purple> | ||
*Digest LasR_MRV with BbsI | *Digest LasR_MRV with BbsI | ||
*{| | *{| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume (uL)''' | |||
|- | |- | ||
| DNA (500ng) || 1.5 | | DNA (500ng)||1.5 | ||
|- | |- | ||
| 10x Green FD Buffer || 2.0 | | 10x Green FD Buffer||2.0 | ||
|- | |- | ||
| BbsI || 1.0 | | BbsI||1.0 | ||
|- | |- | ||
| Roche SAP || 1.0 | | Roche SAP||1.0 | ||
|- | |- | ||
| Water || 14.5 | | Water||14.5 | ||
|- | |- | ||
| Total || 20 | | Total||20 | ||
|} | |} | ||
</font> | </font> | ||
Revision as of 16:47, 1 July 2016
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06/28/2016
|
Reagent | Volume (uL) | |
| DNA (500ng) | 1.5 | ||
| 10x Green FD Buffer | 2.0 | ||
| BbsI | 1.0 | ||
| Roche SAP | 1.0 | ||
| Water | 14.5 | ||
| Total | 20 | ||
|}
