Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/02/09
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02/09/2016
Examining pLas + LasR Ligation Plates Jiaqi performed ligations with pLas and LasR, and after overnight incubation at 37°C, the plates were as shown in the picture below: Figure 1 - pLas + LasR Plates
The plates exhibited the following distribution of colonies:
Generally speaking, there were many satellite colonies that grew around the larger, major colonies.
The general mixture for sequencing consists of...
The mixtures containing the positive control were prepared as shown in the table below:
The sequencing mixture numbers given in the table above correspond to the following plasmids:
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