Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/03: Difference between revisions
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:Incubate at 37°C for 15 minutes. <br> | :Incubate at 37°C for 15 minutes. <br> | ||
:Heat inactivate at | :Heat inactivate at | ||
*80°C for 5 minutes | :*80°C for 5 minutes | ||
*65°C for 20 minutes | :*65°C for 20 minutes | ||
<b> 2. Digest PCR amplified and purified, regulators w/ E+X. </b> | <b> 2. Digest PCR amplified and purified, regulators w/ E+X. </b> |
Revision as of 17:51, 3 July 2015
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06/3/2015
Ligate modular receiver vector (MRV) + RhlR, LuxR, and EsaR.1. Digest 1ug MRV
2. Digest PCR amplified and purified, regulators w/ E+X.
3. Ligate 3:1 ratio of insert:bb
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