Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/03: Difference between revisions
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Vector is ~3,200bp. Transcription factor inserts are ~851bp. <br> | Vector is ~3,200bp. Transcription factor inserts are ~851bp. <br> | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''Reagent''' | ||
| align="center" style="background:#f0f0f0;"|'''Volume (uL)''' | | align="center" style="background:#f0f0f0;"|'''Volume (uL)''' | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
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| Total ||20uL||20uL||20uL||20uL | | Total ||20uL||20uL||20uL||20uL | ||
|} | |} | ||
<br> | |||
<b> 4. Transformation with DH5αT cells </b> <br> | |||
10uL of each ligation reaction were used in the transformation. | |||
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Revision as of 17:46, 3 July 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/3/2015
Ligate modular receiver vector (MRV) + RhlR, LuxR, and EsaR.
Incubate at 37°C for 15 minutes. 2. Digest PCR amplified and purified, regulators w/ E+X.
3. Ligate 3:1 ratio of insert:bb
Amounts of DNA used were based on vector/insert size.
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