Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/01: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2015/07/01 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
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== | ==07/01/2015== | ||
1000μM | |||
'''Testing RhlR with 100μM synthetic N-buteryl-HHL (C4-HSL)''' | |||
:Diluted 1μL of 5mM C4-HSL into 99μL LB. | |||
:Put 2mL LB +amp into 14mL round bottom tube | |||
:Added 4μL diluted C4-HSL for a final concentration of 100μM C4-HSL | |||
:Added 100μL overnight culture of RhlR | |||
:Shook overnight | |||
<br><br> | |||
'''Testing EsaR with 1000μM synthetic N-(3-oxohexanoyl)-HSL (aka N-(β-Ketocaproyl)-L-homoserine lactone aka 3O-C6-HSL)''' | |||
:Diluted 1μL of 10mM C4-HSL into 99μL LB. | |||
:Put 2mL LB +amp into 14mL round bottom tube | |||
:Added 20μL diluted C4-HSL for a final concentration of 100μM C4-HSL | |||
:Added 100μL overnight culture of RhlR | |||
:Shook overnight | |||
Revision as of 09:37, 2 July 2015
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07/01/20151000μM Testing RhlR with 100μM synthetic N-buteryl-HHL (C4-HSL)
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