Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/01: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) (Autocreate 2015/07/01 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
(fix raw html notebook nav) |
||
(4 intermediate revisions by 2 users not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==07/01/2015== | ||
'''Testing RhlR with 100μM synthetic N-buteryl-HHL (C4-HSL)''' | |||
:Diluted 1μL of 5mM C4-HSL into 99μL LB. | |||
:Put 2mL LB +amp into 14mL round bottom tube | |||
:Added 4μL diluted C4-HSL for a final concentration of 100μM C4-HSL | |||
:Added 100μL overnight culture of RhlR | |||
:Shook overnight | |||
<br><br> | |||
'''Testing EsaR with 1000μM synthetic N-(3-oxohexanoyl)-HSL (aka N-(β-Ketocaproyl)-L-homoserine lactone aka 3O-C6-HSL)''' | |||
:Diluted 1μL of 10mM C4-HSL into 99μL LB. | |||
:Put 2mL LB +amp into 14mL round bottom tube | |||
:Added 20μL diluted C4-HSL for a final concentration of 100μM C4-HSL | |||
:Added 100μL overnight culture of RhlR | |||
:Shook overnight | |||
<br><Br> | |||
<font color = purple> | |||
PCR pEsaR and LasR. <br> | |||
Ran gel for colony PCR of RhlI colonies. None of the colonies worked; next step is to pick more (since there were a lot on the plate) and try again before abandoning the batch. | |||
Latest revision as of 01:02, 27 September 2017
Project name | Main project page Previous entry Next entry |
07/01/2015Testing RhlR with 100μM synthetic N-buteryl-HHL (C4-HSL)
PCR pEsaR and LasR.
|