Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/26: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 8: | Line 8: | ||
==05/26/2015== | ==05/26/2015== | ||
<font color = purple> | <font color = purple> | ||
Sequencing results showed a chunk missing from the CerI plasmid, and Ryan realized that the original synthase insert had two EcoRI cut sites. Only one part got ligated into the plasmid, hence the missing segment that sequencing results show. So, we tried a ligation again and this time we only cut with EcoRI. Then, I transformed. We will have to pick more than the usual amount of colonies to see if we can find one with both parts ligated in. | |||
</font> | </font> | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 17:49, 28 May 2015
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
05/26/2015Sequencing results showed a chunk missing from the CerI plasmid, and Ryan realized that the original synthase insert had two EcoRI cut sites. Only one part got ligated into the plasmid, hence the missing segment that sequencing results show. So, we tried a ligation again and this time we only cut with EcoRI. Then, I transformed. We will have to pick more than the usual amount of colonies to see if we can find one with both parts ligated in. | |
