Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==05//2015== | ==05/26/2015== | ||
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Sequencing results showed a chunk missing from the CerI plasmid, and Ryan realized that the original synthase insert had two EcoRI cut sites. Only one part got ligated into the plasmid, hence the missing segment that sequencing results show. So, we tried a ligation again and this time we only cut with EcoRI. Then, I transformed. We will have to pick more than the usual amount of colonies to see if we can find one with both parts ligated in. | |||
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Latest revision as of 00:59, 27 September 2017
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05/26/2015Sequencing results showed a chunk missing from the CerI plasmid, and Ryan realized that the original synthase insert had two EcoRI cut sites. Only one part got ligated into the plasmid, hence the missing segment that sequencing results show. So, we tried a ligation again and this time we only cut with EcoRI. Then, I transformed. We will have to pick more than the usual amount of colonies to see if we can find one with both parts ligated in. | |
