Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/04/21: Difference between revisions

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Digest verification of RpaI colony 5 showed that the plasmid is not what we think it is. <br>
Digest verification of RpaI colony 5 showed that the plasmid is not what we think it is. <br>


[[Image:RpaI Gel Image New.jpg]]
[[Image:RpaI Gel Image New.jpg|600px]] <br>
 
We would expect to see an addition band at ~800bp in the digested lane if the cloning worked. <br><br>




Latest revision as of 00:56, 27 September 2017

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04/21/2015

Digest verification of RpaI colony 5 showed that the plasmid is not what we think it is.


We would expect to see an addition band at ~800bp in the digested lane if the cloning worked.



Next step is to retry with a new digestion with new alkaline phosphatase.



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