Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/04/14: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==04//2015== | ==04/14/2015== | ||
<font color = purple> | |||
I ran a digest verification of Ryan's receiver [[https://benchling.com/s/VFNzuauP/edit| plasmid]] with EcoRI and KpnI<br> | |||
Ryan cloned in an inducible promoter (digestion at BbsI site) and if it worked, it should remove the Kpnl cut site, resulting in one large band around ~3200 <Br> | |||
[[Image:14.4.15 Las inducible promoter gel image .tiff|x300px]] <br> | |||
The gel image shows two bands at around ~2200 and ~1000, which indicates that the inducible promoter did not clone in properly and the Kpnl cut site was still there. <br><Br> | |||
I also ran 3 ligations with: EsaI, RpaI, and RhiI with the gel-purified backbone and alkaline phosphatase treated inserts. <br> | |||
(3:1 ratio, 48ng insert per 50ng backbone). <br> | |||
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Latest revision as of 00:54, 27 September 2017
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04/14/2015
I ran a digest verification of Ryan's receiver [plasmid] with EcoRI and KpnI I also ran 3 ligations with: EsaI, RpaI, and RhiI with the gel-purified backbone and alkaline phosphatase treated inserts. |