Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2015/03/05 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
(fix raw html notebook nav)
 
(6 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==02//2015==
==03/05/2015==
 
<font color = purple>
Today I am doing a digestion of the KAH 126 plasmid that was mini-prepped on 1/28/2015 (in CRISPR notebook). <Br>
Here are the contents used: <br>
<b> Reaction 1</b> <br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Thing'''
| align="center" style="background:#f0f0f0;"|'''Vol'''
|-
| 0.5ug Plasmid|| 1.1 uL
|-
| EcoRI||0.5 uL
|-
| PstI||0.5 uL
|-
| Green digest buffer||1 uL
|-
| Water||6.9 uL
|}
<br>
<b>Reaction 2</b> <br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Thing'''
| align="center" style="background:#f0f0f0;"|'''Vol'''
|-
| 0.5ug Plasmid|| 1.23 uL
|-
| EcoRI||0.5 uL
|-
| PstI||0.5 uL
|-
| Green digest buffer||1 uL
|-
| Water||6.77 uL
|}


<br>


After pipetting each thing into a PCR tube, I ran the product on a gel to see if there was any cutting. This will allow us to conclude whether or not the plasmid is what we think it is. <br>
[[Image:3.5.15 gel digest.png]]
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Latest revision as of 00:48, 27 September 2017

Project name Main project page
Previous entry      Next entry

03/05/2015

Today I am doing a digestion of the KAH 126 plasmid that was mini-prepped on 1/28/2015 (in CRISPR notebook).
Here are the contents used:
Reaction 1

Thing Vol
0.5ug Plasmid 1.1 uL
EcoRI 0.5 uL
PstI 0.5 uL
Green digest buffer 1 uL
Water 6.9 uL


Reaction 2

Thing Vol
0.5ug Plasmid 1.23 uL
EcoRI 0.5 uL
PstI 0.5 uL
Green digest buffer 1 uL
Water 6.77 uL


After pipetting each thing into a PCR tube, I ran the product on a gel to see if there was any cutting. This will allow us to conclude whether or not the plasmid is what we think it is.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs/2015/03/05&oldid=1016457"