Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/26

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02/26/2015

Since the sequencing results failed, we decided to troubleshoot and find out whether our error is with the primers or the plasmid.
I performed a digestion again with XbaI and PstI. I created a reaction for each plasmid sample that I mini prepped on Monday.
Reactions are as follows:

Thing Vol
0.25ug DNA sample 7.5 uL
XbaI 0.5 uL
PstI 0.5 uL
Green digest buffer 2 uL
Water 9.5 uL


After pipetting contents into each tube, I incubated the reactions for 15 minutes at 37°C.
The next step is to run a gel with each reaction and see if we get the desired results.


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