Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/20: Difference between revisions
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Based on the results of the gel we ran, there is reason to believe that the ligation worked in some of the colonies. So, I picked colonies 6, 8, 9, and 10. 6 and 8 are from the 3 | Based on the results of the gel we ran, there is reason to believe that the ligation worked in some of the colonies. So, I picked colonies 6, 8, 9, and 10. 6 and 8 are from the 1:3 plate and 9 and 10 are from the 3:1 plate. I put them in liquid cultures and placed them in the shaker overnight to grow. | ||
Revision as of 14:00, 24 February 2015
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02/20/2015Based on the results of the gel we ran, there is reason to believe that the ligation worked in some of the colonies. So, I picked colonies 6, 8, 9, and 10. 6 and 8 are from the 1:3 plate and 9 and 10 are from the 3:1 plate. I put them in liquid cultures and placed them in the shaker overnight to grow.
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