Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/20: Difference between revisions

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==02/20/2015==
==02/20/2015==
<font color = purple>  
<font color = purple>  
Based on the results of the gel we ran, there is reason to believe that the ligation worked in some of the colonies. So, I picked colonies 6, 8, 9, and 10. 6 and 8 are from the 3:1 plate and 9 and 10 are from the 1:3 plate. I put them in liquid cultures and placed them in the shaker overnight to grow.  
Based on the results of the gel we ran, there is reason to believe that the ligation worked in some of the colonies. So, I picked colonies 6, 8, 9, and 10. 6 and 8 are from the 1:3 plate and 9 and 10 are from the 3:1 plate. I put them in liquid cultures and placed them in the shaker overnight to grow.  
 
[[Image:Wu gel ligation.png]]




Latest revision as of 00:47, 27 September 2017

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02/20/2015

Based on the results of the gel we ran, there is reason to believe that the ligation worked in some of the colonies. So, I picked colonies 6, 8, 9, and 10. 6 and 8 are from the 1:3 plate and 9 and 10 are from the 3:1 plate. I put them in liquid cultures and placed them in the shaker overnight to grow.



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