Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/03: Difference between revisions

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(Autocreate 2015/02/03 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==02/03/2015==
* Insert content here...
<font color = "purple">
I added enzymes to my insert sample (which was PCR-purified the day before) for digestion. I first added water to a 200μL PCR tube, then I added the sample, buffer, and two enzymes.
 
<br>
Restriction digest
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Thing'''
| align="center" style="background:#f0f0f0;"|'''vol in ul'''
|-
| 1ug sample||9.17
|-
| H2O||6.83
|-
| 10x buffer||2
|-
| PstI||1
|-
| BbsI||1
|-
| Total||20
|}
<br>
Purified with PCR clean up kit
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
|-
| insert for ryan||0.012||0.007||1.62||12.1
|}
</font>
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Revision as of 13:46, 6 February 2015

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02/03/2015

I added enzymes to my insert sample (which was PCR-purified the day before) for digestion. I first added water to a 200μL PCR tube, then I added the sample, buffer, and two enzymes.


Restriction digest

Thing vol in ul
1ug sample 9.17
H2O 6.83
10x buffer 2
PstI 1
BbsI 1
Total 20


Purified with PCR clean up kit

Sample Read# 260 280 260/280 ng/µL
insert for ryan 0.012 0.007 1.62 12.1





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