Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/10/02: Difference between revisions

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|-
|-
| ddH2O||32.5
| ddH2O||32.5
|-
| HF buffer||10
| HF buffer||10
|-
| primer F (10uM)||2.5
| primer F (10uM)||2.5
|-
| primer R (10uM)||2.5
| primer R (10uM)||2.5
|-
| dNTP (10mM)||1
| dNTP (10mM)||1
|-
| Template K084007||0.5
| Template K084007||0.5
|-
| Phusion polymerase ||0.5
| Phusion polymerase ||0.5
 
|-
|}
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Revision as of 13:26, 2 February 2015

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mm/dd/yyyy

  • Ran PCR of golden gate parts using Rene's primers using Phusion polymerase
Reagent uL
ddH2O 32.5
HF buffer 10
primer F (10uM) 2.5
primer R (10uM) 2.5
dNTP (10mM) 1
Template K084007 0.5
Phusion polymerase 0.5




Number Template F Primer R Primer Annealing T Expected length
1 K084007 3/10/13 4-2-1 P069 P070 32 645
2 K084007 3/10/13 4-2-2 P069 P070 32 645



  • No clear bands observed.


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