Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/10/02: Difference between revisions
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* | * Ran PCR of golden gate parts using Rene's primers using Phusion polymerase | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | |||
| align="center" style="background:#f0f0f0;"|'''uL''' | |||
|- | |||
| ddH2O||32.5 | |||
| HF buffer||10 | |||
| primer F (10uM)||2.5 | |||
| primer R (10uM)||2.5 | |||
| dNTP (10mM)||1 | |||
| Template K084007||0.5 | |||
| Phusion polymerase ||0.5 | |||
{| {{table}} | {| {{table}} | ||
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* No clear bands observed. | |||
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Revision as of 13:25, 2 February 2015
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