Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/28: Difference between revisions

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(Autocreate 2014/05/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==mm/dd/yyyy==
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Set up golden gate reactions for RhlR receiver with different concentrations of backbone to try to get as close to 0 colonies on the backbone control plates
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Label'''
| align="center" style="background:#f0f0f0;"|'''Bb concentration'''
| align="center" style="background:#f0f0f0;"|'''Bb ul'''
| align="center" style="background:#f0f0f0;"|'''RhlR ul'''
| align="center" style="background:#f0f0f0;"|'''pRhl ul'''
| align="center" style="background:#f0f0f0;"|'''BsmBI'''
| align="center" style="background:#f0f0f0;"|'''T4 Ligase'''
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
|-
| 1||20 fmol||0.74||1.68||2.78||0.5||1.25||2||1.05
|-
| 2||10 fmol||0.37||1.68||2.78||0.5||1.25||2||1.42
|-
| 3||2 fmol||0.74 of 1:10 dil||1.68||2.78||0.5||1.25||2||1.79
|-
| 4||20 fmol||0.74||0||0||0.5||1.25||2||5.51
|-
| 5||10 fmol||0.37||0||0||0.5||1.25||2||5.88
|-
| 6||2 fmol||0.74 of 1:10 dil||0||0||0.5||1.25||2||6.25
|}




Revision as of 15:24, 29 May 2014

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mm/dd/yyyy

Set up golden gate reactions for RhlR receiver with different concentrations of backbone to try to get as close to 0 colonies on the backbone control plates

Label Bb concentration Bb ul RhlR ul pRhl ul BsmBI T4 Ligase T4 Ligase buffer H2O
1 20 fmol 0.74 1.68 2.78 0.5 1.25 2 1.05
2 10 fmol 0.37 1.68 2.78 0.5 1.25 2 1.42
3 2 fmol 0.74 of 1:10 dil 1.68 2.78 0.5 1.25 2 1.79
4 20 fmol 0.74 0 0 0.5 1.25 2 5.51
5 10 fmol 0.37 0 0 0.5 1.25 2 5.88
6 2 fmol 0.74 of 1:10 dil 0 0 0.5 1.25 2 6.25



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