Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/28

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(Autocreate 2014/05/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (18:24, 29 May 2014) (view source)
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Set up golden gate reactions for RhlR receiver with different concentrations of backbone to try to get as close to 0 colonies on the backbone control plates
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Label'''
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| align="center" style="background:#f0f0f0;"|'''Bb concentration'''
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| align="center" style="background:#f0f0f0;"|'''Bb ul'''
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| align="center" style="background:#f0f0f0;"|'''RhlR ul'''
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| align="center" style="background:#f0f0f0;"|'''pRhl ul'''
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| align="center" style="background:#f0f0f0;"|'''BsmBI'''
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| align="center" style="background:#f0f0f0;"|'''T4 Ligase'''
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| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
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| align="center" style="background:#f0f0f0;"|'''H2O'''
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|-
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| 1||20 fmol||0.74||1.68||2.78||0.5||1.25||2||1.05
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|-
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| 2||10 fmol||0.37||1.68||2.78||0.5||1.25||2||1.42
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|-
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| 3||2 fmol||0.74 of 1:10 dil||1.68||2.78||0.5||1.25||2||1.79
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|-
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| 4||20 fmol||0.74||0||0||0.5||1.25||2||5.51
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|-
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| 5||10 fmol||0.37||0||0||0.5||1.25||2||5.88
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|-
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| 6||2 fmol||0.74 of 1:10 dil||0||0||0.5||1.25||2||6.25
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|}

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mm/dd/yyyy

Set up golden gate reactions for RhlR receiver with different concentrations of backbone to try to get as close to 0 colonies on the backbone control plates

Label Bb concentration Bb ul RhlR ul pRhl ul BsmBI T4 Ligase T4 Ligase buffer H2O
120 fmol0.741.682.780.51.2521.05
210 fmol0.371.682.780.51.2521.42
32 fmol0.74 of 1:10 dil1.682.780.51.2521.79
420 fmol0.74000.51.2525.51
510 fmol0.37000.51.2525.88
62 fmol0.74 of 1:10 dil000.51.2526.25



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