Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/22

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Going to run golden gate anyway. Backbone plate should have the same number of PCR template transformants as any of the ligation plates.  
Going to run golden gate anyway. Backbone plate should have the same number of PCR template transformants as any of the ligation plates.  
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200fmol of insert, 20fmol backbone
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Revision as of 14:12, 22 May 2014

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PCR clean up of EsaI PCR and mCh Sender Intermediate for golden gate. Forgot to do DpnI digest of mCh (don't need to for EsaI because the PCR template is kan resistance).

Sample 260 280 260/280 ng/µL
mCh Sender int gg0.0550.0311.77455.337
EsaI0.0460.0261.77645.692



Going to run golden gate anyway. Backbone plate should have the same number of PCR template transformants as any of the ligation plates.
200fmol of insert, 20fmol backbone

Reaction Rec Bb LuxI BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl0.7700.51.25215.48
RhlI0.773.20.51.25212.28
LasI0.772.80.51.25212.68
EsaI0.771.90.51.25213.58


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