Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/22: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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Going to run golden gate anyway. Backbone plate should have the same number of PCR template transformants as any of the ligation plates. | Going to run golden gate anyway. Backbone plate should have the same number of PCR template transformants as any of the ligation plates. | ||
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200fmol of insert, 20fmol backbone | |||
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| align="center" style="background:#f0f0f0;"|'''Reaction''' | | align="center" style="background:#f0f0f0;"|'''Reaction''' |
Revision as of 11:12, 22 May 2014
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mm/dd/yyyyPCR clean up of EsaI PCR and mCh Sender Intermediate for golden gate. Forgot to do DpnI digest of mCh (don't need to for EsaI because the PCR template is kan resistance).
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