Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/21

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(Autocreate 2014/05/21 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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Golden gate 1:10 RhlR and EsaR receivers grew but there were colonies on the backbone plate
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ligation'''
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| align="center" style="background:#f0f0f0;"|'''Colonies'''
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|-
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| Bb||13
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|-
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| RhlR||23
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|-
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| EsaR||14
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|}
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<br><br>
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Ran PCR, extension time 3:30 at 50°C
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Template'''
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| align="center" style="background:#f0f0f0;"|'''F primer'''
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| align="center" style="background:#f0f0f0;"|'''R primer'''
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|-
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| pTet-mCh||130||97
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|-
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| LuxI GFP rec||131||132
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|-
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| LuxR 1:10 colony 1||103||104
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|-
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| LuxR 1:10 colony 2||103||104
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|-
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| LuxR 1:10 colony 3||103||104
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|-
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| LuxR 1:10 colony 4||103||104
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|-
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| LuxR 1:10 colony 5||103||104
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|-
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| LuxR 1:10 colony 5||103||107
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|}
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<br>
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Ran above PCR on gel
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<br><br>
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picked LuxI and RhlI GFP senders into 4mLs media and shook overnight to screen for successful receiver transformations.  

Revision as of 19:06, 22 May 2014

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mm/dd/yyyy

Golden gate 1:10 RhlR and EsaR receivers grew but there were colonies on the backbone plate

Ligation Colonies
Bb13
RhlR23
EsaR14



Ran PCR, extension time 3:30 at 50°C

Template F primer R primer
pTet-mCh13097
LuxI GFP rec131132
LuxR 1:10 colony 1103104
LuxR 1:10 colony 2103104
LuxR 1:10 colony 3103104
LuxR 1:10 colony 4103104
LuxR 1:10 colony 5103104
LuxR 1:10 colony 5103107


Ran above PCR on gel



picked LuxI and RhlI GFP senders into 4mLs media and shook overnight to screen for successful receiver transformations.



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