Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==mm/dd/yyyy==
==mm/dd/yyyy==
All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel.
All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel. If the ligation was successful and BstBI worked, will have bands
 
<br><br>
Transformed golden gate reactions of RhlR and EsaR. Positive transformation control was LuxI sender. 1:1000.
<br><br>


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 15:22, 20 May 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yyyy

All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel. If the ligation was successful and BstBI worked, will have bands

Transformed golden gate reactions of RhlR and EsaR. Positive transformation control was LuxI sender. 1:1000.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs/2014/05/20&oldid=794289"