Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/20

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Current revision (18:22, 20 May 2014) (view source)
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All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel.
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All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel. If the ligation was successful and BstBI worked, will have bands
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Transformed golden gate reactions of RhlR and EsaR. Positive transformation control was LuxI sender. 1:1000.
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All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel. If the ligation was successful and BstBI worked, will have bands

Transformed golden gate reactions of RhlR and EsaR. Positive transformation control was LuxI sender. 1:1000.


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