Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/20

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(Autocreate 2014/05/20 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel.

Revision as of 17:54, 20 May 2014

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All 6 colonies picked from yesterday's LuxR receiver 1:10 plate grew in liquid media and grew back on the plate (I left the plates out on the bench overnight). Miniprepped them all and only one had good concentration and good 260/280. Cut that one with ApaLI and BstBI in FastDigest green buffer for 15 minutes. Not sure if BstBI will work. Cameron ran the digest on the gel.



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